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THE™ GST Antibody, mAb, Mouse
產(chǎn)品簡介:

THE™ Anti-GST Monoclonal Antibody (Mouse) recognizes GST localized at the C-terminal, N-terminal, and internal region of GST-tagged fusion proteins.

產(chǎn)品型號:A00865

更新時間:2025-11-06

廠商性質(zhì):代理商

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產(chǎn)品介紹
產(chǎn)品概述
SpecificityTHE™ Anti-GST Monoclonal Antibody (Mouse) recognizes GST localized at the C-terminal, N-terminal, and internal region of GST-tagged fusion proteins. 
Host SpeciesMouse
ImmunogenRecombinant GST protein (26 kDa)
ConjugateUnconjugated


應用指導
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ApplicationRecommended Usage
ELISA0.05-0.2 µg/ml
Western Blot0.1-1.0 µg/ml
Immunoprecipitation (IP)1 µg/ml
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產(chǎn)品屬性
FormLyophilized
Storage Buffer1: 40 µg, 100 µg, 200 µg (GenScript, A00865), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
2: 500 µg (GenScript, A00865), lyophilized with PBS, pH 7.4, without sodium azide or carrier protein. 
ReconstitutionReconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. 
Storage InstructionsThe product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles.
PurificationProtein A affinity column
IsotypeMouse IgG1,κ
ClonalityMonoclonal
Clone ID2F10B9
NoteGenScript can offer this product per customer's request including product size, buffer components, etc.
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技術背景
Target BackgroundSchistosoma japonicum glutathione S-transferase (GST) is a 26 kDa enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E.coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
SynonymsMouse Anti-GST monoclonal antibody


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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